鈣網(wǎng)蛋白(CALR)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Calreticulin (CALR)
CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60
- 編號(hào)KSB486Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍31.25-2000pg/mL
- 可適靈敏度14.2pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7798¥ 12996¥ 23393¥ 45486¥ 77976
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)鈣網(wǎng)蛋白(CRT)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB486Ra01 | 鈣網(wǎng)蛋白(CALR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB486Ra01 | 鈣網(wǎng)蛋白(CALR)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra22 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra29 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra24 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra25 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra27 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra23 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra21 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC |
| MAB486Ra28 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB486Ra26 | 鈣網(wǎng)蛋白(CALR)單克隆抗體 | ICC/IF |
| SEB486Ra | 鈣網(wǎng)蛋白(CALR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB486Ra | 鈣網(wǎng)蛋白(CALR)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB486Ra | 鈣網(wǎng)蛋白(CALR)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB486Ra01 | 鈣網(wǎng)蛋白(CALR)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Investigative Dermatology | Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes[Pubmed: 23771121] |
| International Journal of Molecular Sciences | The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC[Mdpi: Source] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes[Pubmed:26953684] |
| journal of maternal-fetal & neonatal medicine | Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.[pubmed:28152632] |
| Talanta | Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling[Doi: 10.1016/j.talanta.2018.11.098] |
| Sci Adv | Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia[Pubmed:35171673] |