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腎足蛋白(NPHN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Nephrin (NPHN)

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

  • 腎足蛋白(NPHN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 腎足蛋白(NPHN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對腎足蛋白(NPHN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號適用物種:Rattus norvegicus (Rat,大鼠)應用(僅供研究使用,不用于臨床診斷!)
RPA937Ra01腎足蛋白(NPHN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA937Ra01腎足蛋白(NPHN)多克隆抗體WB; IHC; ICC; IP.
MAA937Ra21腎足蛋白(NPHN)單克隆抗體WB; IHC; ICC; IP.
SEA937Ra腎足蛋白(NPHN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA937Ra腎足蛋白(NPHN)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA937Ra01腎足蛋白(NPHN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
Yonsei medical journalThe Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia.[pubmed:28120572]
Experimental and molecular pathologyAlteration of Connexin43 expression in a rat model of obesity-related glomerulopathy.[pubmed:29246788]
British Journal of Haematology?Renal protection by atorvastatin in a murine model of sickle cell nephropathy[Pubmed:29527679]
Journal of Clinical MedicineMultiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease[Pubmed: 32961903]
Disease MarkersUrinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study[34707724]
Biomed RepEarly biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs[Pubmed:35620307]