離子化鈣結合適配分子1(AIF1)多克隆抗體
Polyclonal Antibody to Allograft inflammatory factor 1 (AIF1)
IBA1; IRT1; Daintain; Protein G1; Ionized calcium-binding adapter molecule 1; G1
- 編號PAC288Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPC288Hu01-離子化鈣結合適配分子1(AIF1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 443¥ 1033¥ 1476¥ 3690¥ 14760
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產品包裝(模擬)
產品包裝(模擬)
DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human AIF1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human AIF1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Colon Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Cerebrum Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Small intestine Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Lymph node Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Lung Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Spleen Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Human Placenta Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Colon Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Cerebrum Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Small intestine Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Lymph node Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Lung Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Porcine Spleen Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant AIF1, Human.
Western Blot; Sample: Lane1: Porcine Spleen lysate; Lane2: Mouse Spleen lysate; Lane3: Rat Spleen lysate<br/>Primary Ab: 0.2μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Cerebellum lysate; Lane3: Porcine Spleen lysate<br/>Primary Ab: 0.3μg/ml Rabbit Anti-Human AIF1 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Human THP1 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human AIF1 Polyclonal Antibody (Catalog PAC288Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對AIF1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別AIF1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
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| RPC288Hu01 | 離子化鈣結合適配分子1(AIF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC288Hu02 | 離子化鈣結合適配分子1(AIF1)多克隆抗體 | WB |
| PAC288Hu01 | 離子化鈣結合適配分子1(AIF1)多克隆抗體 | WB; IHC; FCM |
| LAC288Hu71 | 離子化鈣結合適配分子1(AIF1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAC288Hu81 | 離子化鈣結合適配分子1(AIF1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAC288Hu21 | 離子化鈣結合適配分子1(AIF1)單克隆抗體 | IHC |
| MAC288Hu23 | 離子化鈣結合適配分子1(AIF1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC288Hu22 | 離子化鈣結合適配分子1(AIF1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC288Hu24 | 離子化鈣結合適配分子1(AIF1)單克隆抗體 | WB |
| LAC288Hu72 | 離子化鈣結合適配分子1(AIF1)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAC288Hu82 | 離子化鈣結合適配分子1(AIF1)單克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
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參考文獻
| 雜志 | 參考文獻 |
| Biosci Trends. | Allograft inflammatory factor-1 in the pathogenesis of bleomycin-induced acute lung injury[Pubmed:26911661] |
| Cardiovasc Pathol | A novel role for the Krüppel-like factor 14 on macrophage inflammatory response and atherosclerosis development[pubmed:27923151] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …[articles:journal-of-alzheimers-disease] |
| 博士論文 | INSTITUTO DE CIENCIAS BáSICAS Y PRECLíNICAS “VICTORIA DE GIRóN” DEPARTAMENTO DE HISTOLOGíA[] |