70kDa熱休克蛋白1B(HSPA1B)多克隆抗體
Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)
HSP70-2; HSP-A1B; Heat Shock Protein 72
- 編號PAA301Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPA301Mu01-70kDa熱休克蛋白1B(HSPA1B)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 583¥ 1361¥ 1944¥ 4860¥ 19440
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPA1A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Mouse Small intestine Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Mouse Cerebellum Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Mouse Liver Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Mouse Spinal cord Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: Mouse Kidney lysate; Lane3: Mouse Testis lysate Primary Ab: 0.1μg/ml Rabbit Anti-Mouse HSPA1A Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: HepG2 cell lysate; <br/>Primary Ab: 2μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Western Blot; Samples: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Kidney lysate; Lane4: Mouse Testis lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Mouse HSPA1B Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: A549 cell lysate; <br/>Primary Ab: 2μg/ml Rabbit Anti-Mouse HSPA1A Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對HSPA1B的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別HSPA1B。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
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