甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Parathyroid Hormone Related Protein (PTHrP)
PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone
- 編號SCA819Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍1.37-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于0.55pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 4082¥ 5832¥ 26244¥ 49572¥ 408240
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測甲狀旁腺激素相關蛋白(PTHrP)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的甲狀旁腺激素相關蛋白(PTHrP)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 87-98 | 95 |
| EDTA plasma(n=5) | 84-95 | 91 |
| heparin plasma(n=5) | 82-92 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的甲狀旁腺激素相關蛋白(PTHrP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中甲狀旁腺激素相關蛋白(PTHrP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-103% | 78-92% | 85-99% | 95-104% |
| EDTA plasma(n=5) | 94-105% | 79-92% | 79-103% | 83-99% |
| heparin plasma(n=5) | 80-101% | 79-94% | 84-105% | 90-99% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將甲狀旁腺激素相關蛋白(PTHrP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的甲狀旁腺激素相關蛋白(PTHrP)與連接于固相載體上的抗體結合，然后加入生物素化的甲狀旁腺激素相關蛋白(PTHrP)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發(fā)射光強度和樣品中的甲狀旁腺激素相關蛋白(PTHrP)呈正相關。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA819Hu01 | 甲狀旁腺激素相關蛋白(PTHrP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA819Hu21 | 甲狀旁腺激素相關蛋白(PTHrP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA819Hu22 | 甲狀旁腺激素相關蛋白(PTHrP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA819Hu32 | 甲狀旁腺激素相關蛋白(PTHrP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA819Hu31 | 甲狀旁腺激素相關蛋白(PTHrP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA819Hu01 | 甲狀旁腺激素相關蛋白(PTHrP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA819Hu08 | 甲狀旁腺激素相關蛋白(PTHrP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA819Hu81 | 甲狀旁腺激素相關蛋白(PTHrP)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA819Hu71 | 甲狀旁腺激素相關蛋白(PTHrP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA819Hu22 | 甲狀旁腺激素相關蛋白(PTHrP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA819Hu | 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA819Hu | 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA819Hu | 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA819Hu | 甲狀旁腺激素相關蛋白(PTHrP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA819Hu11 | 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| International journal of biological sciences | PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition[PubMed: PMC3753447] |
| Investigational New Drugs | Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts[Pubmed: 23673814] |
| Toxicology and Applied Pharmacology | Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment[Pubmed:24463094] |
| Arch Gynecol Obstet. | Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study[Pubmed:24943060] |
| PLoS One | 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss[PubMed: 25859665] |
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| Tumour Biol | Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation.[Pubmed:27312487] |
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| Journal of Orthopaedic Surgery and Research | Identification of a potential gene target for osteoarthritis based on bioinformatics analyses[Pubmed: 32571421] |
| International Immunopharmacology | Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related?…[] |
| Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни[] | |
| Cell Death Dis | Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ?-[33510128] |
| Sci Rep | Effects of whole-body vibration training in a cachectic C26 mouse model[34732809] |
| Acta Physiologica | The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals[Pubmed:35334154] |