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甘露糖結合蛋白(MBL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)

ELISA Kit DIY Materials for Mannose Binding Lectin (MBL)

MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

  • 甘露糖結合蛋白(MBL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)產品包裝(模擬)
  • 甘露糖結合蛋白(MBL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)產品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對甘露糖結合蛋白(MBL)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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參考文獻

雜志參考文獻
American Journal of NephrologyAnalysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. [Karger: 000255439]
Current Eye Research Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma[Ingenta: art00007]
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.[PubMed: 22941511]
Clinical and Experimental ImmunologyUrinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy[PubMed: PMC3406374]
Clin J Am Soc Nephrol. Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN[Pubmed: 24115193]
ACTA TROPICANovel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis[Pubmed: 32827456]
PLoS OneComplement activation profile of patients with primary focal segmental glomerulosclerosis[Pubmed: 32569286]
LIFE SCIENCESThe lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes[34242658]