攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)LMA242Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)1.5h
- 檢測(cè)范圍39.06-40000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于13.02 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格8指標(biāo)數(shù)7指標(biāo)數(shù)6指標(biāo)數(shù)5指標(biāo)數(shù)4指標(biāo)數(shù)3指標(biāo)數(shù)2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-95 | 90 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 91-102 | 95 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 98-105% | 89-103% | 97-104% |
| EDTA plasma(n=5) | 89-105% | 80-103% | 92-105% | 80-92% |
| heparin plasma(n=5) | 88-96% | 78-90% | 99-105% | 95-103% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測(cè)溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測(cè)溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)和未標(biāo)記的攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競(jìng)爭(zhēng)結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA242Mu01 | 攝食抑制因子1(NES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA242Mu01 | 攝食抑制因子1(NES1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA242Mu01 | 攝食抑制因子1(NES1)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA242Mu | 攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA242Mu | 攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA242Mu | 攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA242Mu | 攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Bulletin of Clinical Psychopharmacology | Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder[Psikofarmakoloji: 2043] |
| Progress in Neuro-Psychopharmacology & Biological Psychiatry | High plasma nesfatin-1 level in patients with major depressive disorder[PubMed: 21163317] |
| Endocrine | Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus[SpringerLink: h978088657555w16] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212] |
| Psychoneuroendocrinology. | Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder[PubMed: 22595767] |
| Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats[Ijvst: Source] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Decreased serum nesfatin-1 levels in endometriosis[Pubmed: 24702902] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.[Psikofarmakoloji: Source] |
| Current Pharmaceutical Design | Role of NUCB2/nesfatin-1 as a possible biomarker.[Pubmed: 23537082] |
| Regulatory, Integrative and Comparative Physiology | A role of nesfatin-1/NucB2 in dehydration-induced anorexia[Pubmed:24829503] |
| J Endocrinol Invest. | Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome[Pubmed:24920281] |
| Int J Surg. | The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.[Pubmed:25448651] |
| Hormone molecular biology and clinical investigation | The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers[Pubmed:25581765] |
| Turk J Gastroenterol. | New hormones to predict the severity of gallstone-induced acute pancreatitis[Pubmed:25599787] |
| Respiration | Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?[PubMed: 26088934] |
| J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children[PubMed: 26831553] |
| Bosn J Basic Med Sci | Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome[PubMed: 26295295] |
| Original Article / Orijinal Makale | Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1[Sayilar: 294] |
| Gynecol Endocrinol | Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome[PubMed: 26062107] |
| International Journal of Neuroscience | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*[Pubmed:26872406] |
| 未找到 | Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment[P.1.e.012.pdf] |
| International Journal of Developmental | Serum?nesfatin-1?levels: a?potential?new?biomarker?in?patients?with?subarachnoid?hemorrhage.[pubmed:26872406] |
| Current Pharmaceutical Design | Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease[pubmed:28137218] |
| Arch Endocrinol Metab | Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes[pubmed:28977160] |
| Indian Journal of Psychiatry | Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?[pubmed:28529367] |
| Biomedicine & Pharmacotherapy | Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ[Pubmed:29604590] |
| Biochimica et?Biophysica?Acta | Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling[Pubmed:29627363] |
| BMC Psychiatry | Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients[Pubmed:29615007] |
| Probiotics?and Antimicrobial?Proteins | Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar[Pubmed: 30232744] |
| An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages[] | |
| clinical neurology and neurosurgery | Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels[Pubmed: 32311618] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity[Pubmed: 33027789] |
| Endokrynologia Polska | Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke[33283259] |
| Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder[] |