真核翻譯延伸因子2(EEF2)多克隆抗體
Polyclonal Antibody to Eukaryotic Translation Elongation Factor 2 (EEF2)
EF2; Polypeptidyl-tRNA Translocase
- 編號PAC453Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPC453Hu01-真核翻譯延伸因子2(EEF2)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產品包裝(模擬)
產品包裝(模擬)
DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Uterine fibroids Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
Figure. Western Blot; Sample: Recombinant EEF2, Human.
Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human Raji Cells.
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human EEF2 Polyclonal Antibody (Catalog # PAC453Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對EEF2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別EEF2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC453Hu01 | 真核翻譯延伸因子2(EEF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC453Hu01 | 真核翻譯延伸因子2(EEF2)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| FAC453Hu01 | 抗真核翻譯延伸因子2(EEF2)多克隆抗體 | Flow cytometry. |