轉(zhuǎn)酮醇酶(TKT)多克隆抗體
Polyclonal Antibody to Transketolase (TKT)
TK; Wernicke-Korsakoff Syndrome
- 編號PAH019Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPH019Ra01-轉(zhuǎn)酮醇酶(TKT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB,IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Rat Colon Tissue; Primary Ab: 10μg/ml Rabbit Anti-Rat TKT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Liver Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Spinal cord Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat TKT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat TKT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat TKT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant TKT, Rat.
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: TKT knockout Hela cell lysate;
Predicted MW: 68kd
Observed MW: 70kd
Primary Ab: 1μg/ml Rabbit Anti-Rat TKT Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對TKT的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別TKT。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPH019Ra01 | 轉(zhuǎn)酮醇酶(TKT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH019Ra01 | 轉(zhuǎn)酮醇酶(TKT)多克隆抗體 | WB,IHC |
| MAH019Ra21 | 轉(zhuǎn)酮醇酶(TKT)單克隆抗體 | WB; IHC; ICC; IP. |
| SEH019Ra | 轉(zhuǎn)酮醇酶(TKT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH019Ra | 轉(zhuǎn)酮醇酶(TKT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| JOURNAL OF PROTEOME RESEARCH | Discovery of Novel Protein Biomarkers in Urine for Diagnosis of Urothelial Cancer Using iTRAQ Proteomics[33780252] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212] |